Rat MTMR9(Myotubularin Related Protein 9) ELISA Kit

General information

Name

Rat MTMR9(Myotubularin Related Protein 9) ELISA Kit

Size

96T

Catalog number

ELK6476

Price

608 EUR

Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.063ng/mL

Detection range

0.156-10ng/mL

Latin name

Rattus norvegicus

Research Area

Metabolic pathway;

Alternative Names

C8orf9; MTMR8; LIP-STYX

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myotubularin Related Protein 9 (MTMR9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myotubularin Related Protein 9 (MTMR9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myotubularin Related Protein 9 (MTMR9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myotubularin Related Protein 9 (MTMR9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.